[Shandong University] A multi-functional isolated organ experimental device

[A multi-functional isolated organ experimental device]

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Introduction to function:

1. Introduction

The isolated organ/tissue experiment is the most classic and most commonly used experiment in medical experiments. It is an important part of the medical function discipline including the teaching of physiology, pharmacology and pathophysiology. It is also an important part of cultivating students' experimental skills and scientific literacy. However, in the long-term teaching practice, we found that the original isolated organ experimental device has many defects and deficiencies, resulting in difficulty in hanging the isolated specimen, complex tension adjustment, difficult to detect the biological signal, poor experimental results and low success rate. . These problems directly affect the quality of experimental teaching and limit the further improvement of relevant experimental levels.

In response to this problem, we took the "in vitro specimen suspension and biosignal correction adjustment" as the entry point, and introduced the precise "three-dimensional rotation/positioning adjustment mechanism" into the isolated organ experiment, and developed an experimental teaching suitable for medical functional disciplines. The new "multi-functional isolated organ experimental device" further perfects the experimental method of isolated organs, making the suspension and biological signal regulation and detection of the isolated specimens more scientific, accurate and simple, further improving the experimental level and the quality of experimental teaching.

The "Multifunctional Isolated Organ Experiment Device" consists of a vertical rod bracket, a constant temperature bath, a three-dimensional rotation/positioning adjustment device, a tension sensor, and a biosignal detection system. Through the fine adjustment of the three-dimensional angle, the suspension position of the isolated specimen can be quickly determined, the positioning suspension of the isolated specimen can be quickly completed, the biological activity of the isolated tissue can be protected to the greatest extent, and the experimental efficiency and experimental success rate can be improved. Through the fine adjustment of the bidirectional rotation angle, the accurate correction of the specimen suspension angle and the signal guiding direction can be realized, and the accurate adjustment of the resting tension can be used to guide the biological signal more scientifically and reasonably, and the variation thereof can be accurately detected, thereby effectively reducing the experimental error and ensuring The experimental data is accurate and reliable, further improving the experimental level and the quality of experimental teaching.

The device has been applied to five majors (clinical medicine, preventive medicine, stomatology, nursing, pharmacy) and four levels (five-year, seven-year, eight-year) from the first semester of the 2011-2012 school year. Experimental teaching of four courses (physiology, pharmacology, pathophysiology, medical dynamism), more than 1,200 hours of experimental teaching in three years, a total of more than 3,000 students benefited. The teaching practice in the past three years shows that the device is reasonable in design and simple in operation, and can completely solve the problems of difficulty in hanging the isolated specimen, complicated adjustment of resting tension, and difficulty in accurately detecting the biological signal. Through the omnidirectional multi-angle correction adjustment, the positioning suspension of the isolated specimen can be quickly completed, the signal guiding direction can be accurately corrected, the biological signal can be correctly guided and the change can be accurately detected, and the suspension speed of the isolated specimen and the detection accuracy of the biological signal can be greatly improved. It effectively reduces the experimental error, significantly improves the experimental efficiency and the success rate of the experiment, and is highly praised by students and instructors.

The “Multifunctional Isolated Organ Experiment Device” was awarded the “Practical Model Patent Certificate” (Patent Certificate No.: 1991090) issued by the State Intellectual Property Office in November 2011. The device is suspended from the specimen and the biosignal correction is performed. Adjustment, testing and other aspects are at the domestic advanced level.

2. Introduction to function

The isolated organ experiment is a classic experiment in the medical function discipline, and is also the most commonly used experiment. It is an important part of the experimental teaching of medical functional science. The experimental teaching of functional disciplines at all levels of our majors should carry out “in vitro gastrointestinal smooth muscle movement”, “influencing factors of isolated tracheal smooth muscle activity”, and “in vitro frog heart perfusion” and “diphenhydramine antagonistic parameters”. Determination of PA2" aliquot experimental project. Correct suspension of isolated specimens, reasonable guidance of tension signals, accurate adjustment of resting tension, and accurate detection of changes in biological signals are key steps in in vitro experiments and are the main functional link of the device.

The device is applied in the teaching of isolated organs:

(1) Quickly complete the positioning suspension of the isolated specimen: the nutrient solution is injected into the constant temperature bath, the temperature of the nutrient solution is controlled to be 37.2 to 37.5 °C by using the heater and the temperature sensor, and the external ventilating device is connected through the boom to supply oxygen to the isolated specimen. To simulate an artificial environment similar to the animal's internal environment. Hook the ends of the isolated specimen with the "L" type hook and the wire hook diagonally, and place the isolated organ into the nutrient solution in the constant temperature bath. The "L" type hook is fixed to the front end of the horizontal trimmer by a boom, and the wire hook is suspended by the wire at the front end of the tension sensor. Rotating the vertical adjustment knob rotates the gear in the vertical trimmer and meshes with the rack, changing the circular motion of the gear to a linear motion, pushing the vertical trimmer and the horizontal trimmer fixed to the vertical trimmer to achieve vertical vertical Move, and then move the isolated organ up or down by the boom at the front of the horizontal trimmer. Similarly, the rotation horizontal X and Y adjustment knobs can drive the horizontal trimmer to move along the X-axis and Y-axis directions, and then move the isolated organs in the horizontal direction to realize the macro movement in the X-axis and Y-axis directions. With the rotation vertical adjustment knob and the horizontal adjustment knob, the suspension position of the isolated specimen can be quickly determined at the three-dimensional angle, and the positioning suspension of the isolated specimen can be quickly and accurately realized.

(2) Accurately correct the signal guiding direction, correctly guide the biosignal and accurately detect the change: adjust the L-hook fixing rod and the rotating nut of the tension sensor to keep the two rotation at the same angle, and quickly adjust the relative position of the sensor and the isolated specimen. Accurately correct the specimen suspension angle and signal guiding direction to avoid the signal guiding error caused by the specimen twisting and diagonal pulling, and achieve the correct guiding of the tension signal. The tension signal of the isolated organ can be transmitted to the tension sensor through the wire, and the tension sensor converts the collected tension signal into an electrical signal and sends it to an external computer biofunctional experiment system to accurately detect the change of the biological signal.

Name of the work: a multi-functional isolated organ experimental device

Completed by: Shandong University

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